Coding

Part:BBa_J51001:Design

Designed by: Peter Nguyen   Group: iGEM06_Rice   (2006-10-25)


ComA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 12
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 12
    Illegal NgoMIV site found at 426
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: The Biobrick prefix and suffix sites are non-standard! Due to the high percentage of A+Ts in the ComQXPA genes, the primer hybridization sites were lengthened and the Biobrick sites correspondingly shortened (the NotI sites between the E-X and S-P restriction sites was removed).


Primers used for isolation and construction (5'->3')

F: cggaattccctctagATGAAAAAGATACTAGTGATTGATGACCATCCGG

R: gactgcagctactagTTAAAGTACACCGTCTGATTTCGCAATC


NOTE: The ComA part contains an internal SpeI site, which prevents cloning using standard assembly. This will be removed by site-directed mutagenesis in the future.

Source

This part was PCR amplified from B. subtilis 168 genomic DNA.

References